Journal Information
Journal ID (publisher-id): BM
Journal ID (nlm-ta): Biochem Med (Zagreb)
Title: Biochemia Medica
Abbreviated Title: Biochem. Med. (Zagreb)
ISSN (print): 1330-0962
ISSN (electronic): 1846-7482
Publisher: Croatian Society of Medical Biochemistry and Laboratory Medicine
Article Information
Copyright statement: ©Croatian Society of Medical Biochemistry and Laboratory Medicine.
Copyright: 2023, Croatian Society of Medical Biochemistry
License (open-access):
This is an Open Access article distributed under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Date received: 04 April 2024
Date accepted: 06 July 2024
Publication date: 15 October 2024
Publication date: 15 October 2024
Volume: 34
Issue: 3
Electronic Location Identifier: 030704
Publisher ID: bm-34-3-030704
DOI: 10.11613/BM.2024.030704
Short- and long-term stability of synovial fluid calprotectin
Stjepan Bulat[2]
Joško Jeličić[2]
Ksenija Maštrović Radončić[6]
Lora Dukić[8]
[1] Department of Medical Laboratory Diagnostics, University Hospital Sveti Duh, Zagreb, Croatia
[2] Department of Orthopaedic Surgery and Traumatology, University Hospital Sveti Duh, Zagreb, Croatia
[3] School of Medicine, University of Zagreb, Zagreb, Croatia
[4] Department of Clinical Medicine, School of Applied Health Sciences, University of Zagreb, Zagreb, Croatia
[5] Polyclinic Ivković, Zagreb, Croatia
[6] Department of Rheumatology, Physical and Rehabilitation Medicine, University Hospital Sveti Duh, Zagreb, Croatia
[7] Faculty of Kinesiology, University of Zagreb, Zagreb, Croatia
[8] Clinical Department of Laboratory Diagnostics, University Hospital Center Rijeka, Rijeka, Croatia
[9] Faculty of Pharmacy and Biochemistry, University of Zagreb, Zagreb, Croatia
[10] Department of Global Medical & Clinical Affairs, Business Unit for Preanalytics, Greiner Bio-One GmbH, Kremsmünster, Austria
Author notes:
[*] Corresponding author: helena.cicak5@gmail.com
Author contributions
H Čičak: Formal analysis, Investigation, Methodology, Project administration, Validation, Visualisation, Writing - original draft; S Bulat: Formal analysis, Investigation, Methodology, Writing - review & editing; J Jeličić: Formal analysis, Investigation, Methodology, Writing - review & editing; A Ivković: Formal analysis, Investigation, Methodology, Supervision, Writing - review & editing; K Maštrović-Radončić: Formal analysis, Investigation, Methodology, Supervision, Writing - review & editing; V Radišić Biljak: Formal analysis, Investigation, Methodology, Supervision, Writing - review & editing; L Dukić: Formal analysis, Investigation, Methodology, Supervision, Writing - review & editing, Project administration; AM Šimundić: Conceptualization, Methodology, Project administration, Resources, Supervision, Writing - review & editing.
• Synovial calprotectin may have an important role in diagnosing inflammatory joint conditions
• This stability study was done according to the Checklist for Reporting Stability Studies (CRESS) recommendations
• The CRESS recommendations enabled better standardization among stability studies
• Calprotectin in synovial fluid samples is stable for 7 days at 4-8 °C
• Calprotectin in synovial fluid samples is stable for 6 weeks at - 20 °C and - 80 °C
Introduction
Information about analyte stability is of crucial importance. The aims of this study were to determine the short- and long-term stability of synovial fluid calprotectin at various temperature conditions (4-8 °C for 7 days, - 20 °C and - 80 °C for 6 weeks).
Materials and methods
Eleven samples from patients were included in this study. The samples were promptly transported at room temperature (RT) to the laboratory immediately after arthrocentesis. Upon arrival, the samples were transferred into plastic tubes without additives and pretreated with hyaluronidase solution. After centrifugation at 1500xg for 10 minutes at RT, the baseline calprotectin concentrations were determined. Seven aliquots were stored in LoBind tubes (Eppendorf) at 4-8 °C and the calprotectin was measured every day. Six additional aliquots were stored at temperatures - 20 °C and - 80 °C and the concentration of calprotectin was measured weekly. Analysis was done using Buhlmann fCAL turbo reagent on analyzer Siemens Atellica Solution (Siemens Healthcare, Erlangen, Germany). Data were analyzed by Microsoft Excel and MedCalc statistical software. The percentage difference (PD%) was calculated. The maximum permissible difference (MPD) was 9.1% for PD%.
Results
The PD% with the corresponding 95% confidence intervals were inside the predefined MPD. The instability equations and correlation coefficient for storage temperatures were PD% = 0.1644 x time (day), r = 0.06, P = 0.614 for 4-8°C, PD% = 0.5190 x time (week), r = - 0.22, P = 0.080 for - 20°C, and PD% = 0.1316 x time (week), r = 0.08, P = 0.545 for - 80°C.
Keywords: calprotectin; preanalytical phase; stability; synovial fluid
